By Kenneth C. Torres
This e-book was once written for these people who are taken with the ideas and practices of plant mobilephone cultures for horticultural vegetation. It was once designed to function a textual content and reference for college kids and pros in decorative horticulture, fruit and vegetable crop professional duction, botany, forestry, and different components of plant technological know-how. examine over the last twenty-five years within the quarter of plant tissue tradition has ended in many advancements and adjustments during this box. Al although the options desirous about the manipulation of plant tissue tradition are actually really ordinary, the presentation of those suggestions in a quick quantity for the newbie within the box is mostly unavailable. as well as describing the thoughts for institution and manipulation of particular species, numerous chapters during this publication additionally offer a quick, basic evaluate of vital cultural parameters. Spe cific protocols and laboratory tactics can also be present in the appendix. i'm hoping that this presentation of data can be priceless to these participants desirous to follow plant tissue tradition concepts for horticultural crops.
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Extra info for Tissue Culture Techniques for Horticultural Crops
Ohio State Univ. Press, Columbus. Stuart, R. and H. E. Street. 1971. Studies on the growth in culture of plant cells. x. Further studies on the conditioning of culture media by suspensions of Acer pseudoplantanus L. J. Exp. Bot. 20:556-571. , A. Maretzki, E. Komer and W. S. Sakai. 1981. Nutrient uptake and accumulation by sugarcane cell cultures in relation to growth cycle. Plant Cell Tissue and Organ Culture 1:3-14. Thorpe, T. A. 1978. Physiological and biochemical aspects of organogenesis in vitro.
F. Paddock, and V. Raghaven (editors). Ohio State Univ. Press, Columbus. Stuart, R. and H. E. Street. 1971. Studies on the growth in culture of plant cells. x. Further studies on the conditioning of culture media by suspensions of Acer pseudoplantanus L. J. Exp. Bot. 20:556-571. , A. Maretzki, E. Komer and W. S. Sakai. 1981. Nutrient uptake and accumulation by sugarcane cell cultures in relation to growth cycle. Plant Cell Tissue and Organ Culture 1:3-14. Thorpe, T. A. 1978. Physiological and biochemical aspects of organogenesis in vitro.
The various sterilants may be used alone or in sequence to obtain the most effective sterilization procedure. Following disinfestation or sterilization, the explant tissue must be rinsed several times in sterile distilled water to remove any remaining traces of the disinfectant. The damaged tissue ends of the explant are then removed and the explant subdivided into appropriate sizes. The explant is then placed on a nutrient medium designed for maximum growth of that particular species. Plant tissues having internal contaminants can sometimes be disinfected by adding 10 mglliter of benomyl or benlate to the culture medium or by treating the tissue with either benomyl or benlate before disinfestation with commercial bleach.
Tissue Culture Techniques for Horticultural Crops by Kenneth C. Torres