By Egon Stahl (auth.), Prof. Dr. Egon Stahl (eds.)
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Additional resources for Thin-Layer Chromatography: A Laboratory Handbook
00, in ml. The values are reproducible for different samples of one and the same silica gel. A special advantage of the method is that it can be applied also to plastic polysilicic acid gels, i. , the precursor of silica gel. The values for the specific surface area of a plastic polysilicic acid gel and for the corresponding hard xerogel (silica gel) can be closely similar. There is so far no final answer to the question how far agreement exists between the surface area values for a silica gel as obtained by the BET-method and by the titration method.
06 mole HaO/SiOa). 0 Reproducib-ility of Silica Gel Preparations The current status of silica gel research is such that not all properties of the silica gel substance can be quantitatively described. Some can be described only approximately and others are still largely unknown. The real surface structure is one of these last named properties. The more complete our knowledge of the formation, framework and cavity system becomes, the more easily possible it will be to reproduce accurately those properties which are important for the use of silica gel as an adsorbent.
They differ from the usual materials for column chromatography in their fine-grained structure (Fig. 3). The thin layers prepared from these materials and their surprising separating ability, are a major feature of TLC. When choosing the most suitable grain size, technical preparation and migration times on the chromatogram must be considered and a compromise arrived at. The grain size of most TLC adsorbents lies between 5 and 50 [Lm. Efforts must be made also to standardise the adsorbents so that their separation behaviour is as uniform as possible, showing no variations from manufacturer to manufacturer or from charge to charge.
Thin-Layer Chromatography: A Laboratory Handbook by Egon Stahl (auth.), Prof. Dr. Egon Stahl (eds.)