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By Lions J.L., Magenes E.

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The detector system consists of a differential refractometer. The volume of individual fractions is measured exactly into a siphon and then directed to the fraction collector. The entire system - from the application of the sample to the siphon - is temperature adjustable up to 140° C, enabling analysis of poorly soluble plastics to be carried out. This excellent analytical tool is shown in Fig. 11. / / / ~ e Q g-&lLUlli Fig. 11. Schematic representation of the gel permeation chromatograph from Waters Associates.

Table 9). By their very nature, the range of applications of these thermally reversible gels is limited. The gel congeals at elevated temperatures or upon application of eluants which are capable of breaking hydrogen bonds (e. g. urea solutions) and can no longer be used. Growth of bacteria, which would cause clogging of the column, has to be controlled carefully. Preparation of Organophilic Gels Sephadex Derivatives The swelling properties of hydrophilic Sephadex types in polar organic solvents improve with increasing substitution.

The various pieces of equipment described in the preceding section serve only the purpose of increasing accuracy, sensitivity, and convenience. The development of instruments for gas chromatography evidently has had some influence in this field too; some companies manufacture complete chromatography units in which pumps, columns with thermostats, detectors, and collectors are combined into one functional unit. Beckman, for instance, offers in its "Spectrochrom" a complete laboratory installation for liquid chromatography in the aqueous phase.

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Non-Homogeneous Boundary Value Problems and Applications: Vol. 3 by Lions J.L., Magenes E.

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