By W. O. Abel, P. Dörmer, W. Eschrich, E. Fritz, R. G. Herrmann, A. Läuchli, U. Lüttge, J. B. Passioura, J. D. Pickett-Heaps (auth.), U. Lüttge (eds.)
In organic literature, a number of definitions of quantitative autoradio graphy are given. The time period is outlined as both the selection and com parison of the density of silver grains above a variety of constructions or lower than various stipulations, or the choice of absolute amounts of radio task. In either those situations, photometric dimension serves for extra swift and extra certain evaluate of grain densities than will be attainable via visible counting of the grains. The gear mostly used for the photometric dimension of silver grains contains a microscope, a photocell, an digital amplifier procedure and a exhibit unit. Grains might be made obtainable to photometric overview by way of several types of microscopic illumination: 1. Substage bright-field illumination. 2. Substage dark-field illumination. three. Incident dark-field illumination. four. Vertical bright-field illumination. With some of these varieties of illumination, the connection among the luminous flux I absorbed through the movie, scattered into the target and mirrored or diffracted, and the flux 10 which isn't laid low with the movie is used as a degree of grain density. considering the fact that those are differential measurements, the sunshine beam I transmitted by means of the movie is in itself a degree of grain density if the luminous flux 10 incident at the grains is stored consistent. This strategy has been utilized in a lot of measuring setups.
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Extra info for Microautoradiography and Electron Probe Analysis: Their Application to Plant Physiology
8, 113-126 (1959). ROGERS, A. : Techniques of autoradiography. Amsterdam-London-New York: Elsevier 1967. J. B. 5. Appendix: The Computer Programs Note 1. The programs are written in a fairly advanced form of FORTRAN in order to save space. Consequently, they may need minor modifications to run on some computers. Note 2. AUTORAD 1 operates on E (I), S (I) and G (I) only from I = 3 to I = N - 2. Consequently, E (1) and E (2) should be set equal to E (3), and E (N) and E (N - 1) should be set equal to E (N - 2).
Analysis of distribution of Ca 45 in dog bone by a quantitative auto radiographic method. J. Bone Jt. Surg. 47-A, 359-370 (1965). : Die Beziehung zwischen der Schwarzung und der GroBe der entwickelten Silberaggregate. Z. Elektrochem. 62, 993-999 (1958). : Die quantitative Bestimmung der Verteilung von S35-Sulfat bei der Maus mittels Autoradiographie. Nucl. Med. 3, 39-50 (1963). : Untersuchung der Kinetik der Ionenaufnahme in das Zytoplasma von Mnium-Blattzellen mit Hilfe der Mikroautoradiographie und der RontgenMikrosonde.
2, 135-144 (1954). : Quantitative autoradiography using a radioactive wedge. Int. J. Appl. Radiat. 1, 178-183 (1956). MARINELLI, L. , HILL, R. : Radioautography. Some physical and radiobiological aspects of the technique as applied to thin specimens. Amer. J. Roentgeno!. 59, 396-403 (1948). MARSHALL, J. , ROWLAND, R. : Microscopic metabolism of calcium in bone. II. Quantitative autoradiography. Radiat. Res. 10, 213-233 (1959). : GroBe der tJ-Selbstabsorption bei der 3H-Autoradiographie. Exp. Cell Res.
Microautoradiography and Electron Probe Analysis: Their Application to Plant Physiology by W. O. Abel, P. Dörmer, W. Eschrich, E. Fritz, R. G. Herrmann, A. Läuchli, U. Lüttge, J. B. Passioura, J. D. Pickett-Heaps (auth.), U. Lüttge (eds.)