By D Marshall Prescott
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Additional resources for Methods in Cell Biology, 10
J. Cell Biol. 55, 199a. Pardue, M. , Brown, D. , and Birnstiel, M. L. (1973). Uwomosomu 42, 191-203. , Steffensen, D. , and Hughes, W. L. (1973). Proc. Nut. Amd. Sci. S. 70, 1860-1 864. Verma, I. , and Baltimore, D. (1974). In “Nucleic Acids and Protein Synthesis” (L. Grossman and K. ), Methods in Enzymology, Vol. 29, 125-130. Academic Press, New York. Walker, P. M. B. (1969). Progr. Nucl. Acid Res. Mol. Biol. 9, 301-326. Wetmur, J. , and Davidson, N. (1968). J. Mol. Biol. 31, 349-370. Wimber, D.
C . Electrophoresis The quartz glass with the gel strip is transferred to an electrophoretic chamber of the type described by Wieme (1963, but made smaller so that it fits the quartz glass used (Fig. 12). The gel strip forms a bridge between two blocks of 2% agarose in electrophoretic buffer. Each block is connected to +I I 75 mm I - I FIG. 12. Electrophoretic chamber for microelectrophoresis. A quartz glass with a gel strip (containing the applications of RNA samples) is inverted and placed in the electrophoretic chamber.
The low efficiency of hybridization may be a reflection of the availability of the DNA in cytological preparations. However, it is also possible that none of the hybridizations were done with saturating concentrations of nucleic acid in solution. It is unlikely that the concentrations and times now used for in situ hybridization reactions would permit the detection of unique sequences in the DNA of diploid cells of higher organisms. However, unique sequences can easily be studied in polytene chromosomes in which the close alignment of multiple chromatids provides high local concentrations of the sequences.
Methods in Cell Biology, 10 by D Marshall Prescott