Read e-book online Circulating Nucleic Acids in Early Diagnosis, Prognosis and PDF

By Peter B. Gahan

ISBN-10: 9401791678

ISBN-13: 9789401791670

ISBN-10: 9401791686

ISBN-13: 9789401791687

DNA and RNA fractions should be remoted from numerous physique fluids together with entire blood, serum, plasma, urine, saliva and cerebrospinal fluid from either sufferers and fit members. Such isolates could be exploited within the early detection of medical problems, stratification of sufferers for remedy, therapy tracking and scientific follow-up. moreover, the use in fetal medication permits the early detection of fetal intercourse, Rh issue and aneuploid problems in addition to following either fetal and untimely born youngster improvement. This quantity is meant as a primer in the event you have an interest in getting into the sphere of circulating nucleic acids.

The components lined during this quantity include:

· history and basic biology of circulating nucleic acids

· Methodology

· purposes of circulating nucleic acids

· caliber Assurance

· Ethics

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Extra resources for Circulating Nucleic Acids in Early Diagnosis, Prognosis and Treatment Monitoring: An Introduction

Sample text

J Exp Med 79:137–158 5. Watson JD, Crick FHC (1953) A structure for deoxyribose nucleic acid. Nature 171:737–738 6. Wilkins MFH, Stokes AR, Wilson HR (1953) Molecular structure of deoxypentose nucleic acids. Nature 171:738 7. Sinsheimer R (1957) First steps toward a genetic chemistry. Science 125:1123–1128 8. Steinman CR (1975) Free DNA in serum and plasma from normal adults. J Clin Invest 56:512–515 9. Anker P, Stroun M, Maurice P (1975) Spontaneous release of DNA by human blood lymphocytes as shown in an in vitro system.

Autoimmun Rev 3:500–504 42. Atamaniuk J, Ruzicka K, Stuhlmeier KM, Karimi A, Eigner M, Mueller MM (2006) Cell-free plasma DNA: a marker for apoptosis during hemodialysis. Clin Chem 52:523–526 43. Viorritto IC, Nikolov NP, Siegel RM (2007) Autoimmunity versus tolerance: can dying cells tip the balance? Clin Immunol 122:125–134 44. Goebel G, Zitt M, Zitt M, Mu¨ller HM (2005) Circulating nucleic acids in plasma or serum (CNAPS) as prognostic and predictive markers in patients with solid neoplasias.

The mitDNA is rapidly (<1 s) released involving a catapult-like movement. mitDNA, together with protein, forms the extracellular network that is believed to bind and kill bacteria in vitro and in vivo under inflammatory conditions [25]. However, Menegazzi et al. [26] have challenged this explanation suggesting that the DNA network was released only by dying neutrophils. This is based upon experiments in which live bacteria were released from the DNA network on treatment with DNAase. g. [27]). B.

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Circulating Nucleic Acids in Early Diagnosis, Prognosis and Treatment Monitoring: An Introduction by Peter B. Gahan

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